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Written by: Medical Affairs Team

Length: 5 minute read

Posted:

  • Health and Nutrition
  • Mental Health
  • Nutritional Supplements
  • Vitamins & Minerals

When Is the Best Time to Take B Vitamins?

When Is the Best Time to Take B Vitamins?

Laura Lewis, MS

Timing Your MethylPro B-Complex for Maximum Benefit 

If you’re looking to optimize your health and energy levels, B vitamins are a powerhouse group of nutrients to focus on. Essential for everything from energy production to brain function and mood regulation, these water-soluble vitamins work as a team and need to be replenished daily. But timing matters. So, when is the best time to take B vitamins? For most people, the answer is in the morning and after a meal—and here’s why. 

Understanding the B Vitamins in your B-Complex 

B-complex supplements typically include eight key B vitamins, each with a unique and essential role in your body: 

  • Vitamin B1 (Thiamine): Supports the nervous and cardiovascular systems. Works as a coenzyme in cell metabolism and helps convert nutrients (mainly carbohydrates) into energy [1].

  • Vitamin B2 (Riboflavin): Plays a role in numerous biological pathways, mainly as a coenzyme and through its antioxidant activities [2]. Aids in energy production (mitochondrial function), metabolism of other nutrients, and supports skin and oral health, among other benefits [2].

  • Vitamin B3 (Niacin): Supports metabolism, DNA repair, and normal lipid balance [3].

  • Vitamin B5 (Pantothenic Acid): Essential for making coenzyme A, which is vital for fatty acid metabolism and hormone synthesis, among other biochemical pathways [4]. It may also support clear skin, skin integrity, and healthy cholesterol balance [4].

  • Vitamin B6 (Pyridoxine): Antioxidant vitamin with a vital role in metabolism of macronutrients and nucleic acids [5]. Essential for neurotransmitter synthesis, immune function, and homocysteine regulation [5].  

  • Vitamin B7 (Biotin): Known for its role in hair, skin, and nail health, but also important for carbohydrate and fat metabolism, as well as for a healthy nervous system [5].

  • Vitamin B9 (Folate or L-Methylfolate): Supports neurotransmitter synthesis, DNA synthesis, and aids normal homocysteine metabolism in support of the cardiovascular and nervous systems [5]. 

  • Vitamin B12 (Cobalamin): Plays a crucial role in red blood cell formation and DNA synthesis, and helps normal homocysteine metabolism in support of the cardiovascular and nervous systems [5].

These vitamins often work synergistically and are commonly combined into one supplement for overall metabolic and neurological support. 

Why Morning May Be Best 

Most B vitamins, especially B1, B2, B3, B5, and B6, play a direct role in energy metabolism [5]. Taking them in the morning can help support your body’s natural circadian rhythm, help you feel alert and energized in the morning, and promote sustained energy throughout the day. 

B6, B9, and B12 all influence serotonin and dopamine levels, which can be a boon to your morning, but impact your sleep if taken at night. Additionally, Vitamin B12 has stimulating properties [7]. While it doesn’t work like caffeine, it can still increase alertness and mental clarity, which is why taking B vitamins late in the day might interfere with your ability to wind down at night or fall asleep [7]. 

With all of that said, B-vitamins (like most supplements) are most effective when taken at the same time, daily. Because they are safe to take at any time, the “best” time is whenever you know you’ll remember to take them.  

Should I Take B Vitamins with Food? 

B vitamins are water-soluble, which means they’re not stored in the body and must be consumed daily. It also means that they do not require fat to be absorbed, and so can be taken on an empty stomach or with a meal. There are a few caveats, however: 

  • Better B12 Absorption: B12 may be better absorbed with food, as a meal stimulates the release of intrinsic factor, one of the transport proteins necessary for B12 absorption in the gut [8].
  • Comfort: Taking B-complex on an empty stomach can lead to temporary nausea in some people. Specifically, potent dosing of B6, and B12 are known to leave some stomachs unsettled when consumed without food [9,10]. Niacin (B3) is also known to cause skin flushing in some individuals, which can also be mitigated by taking niacin with a meal [11].
  • Enzyme Activation: Many B vitamins function as coenzymes in metabolic reactions that are triggered during digestion, making them more useful when taken with a meal [12].

Why Forms of B Vitamins Matter 

Not all B-complex supplements are created equal. At MethylPro®, we use methylated forms of folate (L-Methylfolate) and B12 (Methylcobalamin), and phosphorylated forms of B3 (Riboflavin 5’ Phosphate) and B6 (Pyridoxal 5’ Phosphate) which are the active, bioavailable forms that your body can use right away—no conversion needed. 

This is especially important for people with MTHFR genetic variations, who may have difficulty converting synthetic folic acid into its usable counterpart, L-Methylfolate 

MethylPro offers advanced, clinical-grade formulations designed to support methylation, a key biochemical pathway that affects everything from mood, to detoxification, to cardiovascular health. 

Final Takeaway: Morning + Meal = Maximum Benefit 

So, when is the best time to take B vitamins? For most people, it’s in the morning with breakfast or your first meal of the day. This timing aligns with your body’s natural energy demands and supports optimal absorption, without disrupting your sleep cycle, or leaving your stomach feeling off. 

Looking to upgrade your daily wellness routine? Try one of our methylated B-complex formulas for peak performance, energy, and mental clarity—delivered in forms your body recognizes and uses with ease. 

Disclaimer:
The information provided is for educational purposes only. Consult your physician or healthcare practitioner if you have specific questions before instituting any changes in your daily lifestyle including changes in diet, exercise, and supplement use.

Laura Lewis is a clinical researcher and science communicator with a Master of Science degree and a background in nutrition science and integrative health. She brings a decade of experience translating complex clinical data into accessible insights for healthcare practitioners and patients alike.

Laura has contributed to educational programming and strategic content development across the integrative and functional medicine space. Her strengths lie in evidence synthesis, practitioner engagement, and crafting communications that bridge the gap between scientific rigor and real-world application. At ARG, Laura supports clinical content development and educational initiatives that elevate practitioner confidence and product credibility.

  1. Bettendorff, Lucien. Present Knowledge in Nutrition. Academic Press, 2020. 171-188. https://www.sciencedirect.com/science/article/abs/pii/B978032366162100010X 
  2. Olfat, N, et al. British Journal of Nutrition 128.10 (2022): 1887-1895. https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/riboflavin-is-an-antioxidant-a-review-update/6434B395E5D41953FDF975198679FF59 
  3. Penberthy, William Todd, and James B. Kirkland. Present knowledge in nutrition. Academic Press, 2020. 209-224. https://www.sciencedirect.com/science/article/abs/pii/B9780323661621000123 
  4. Sanvictores, Terrence, and Shaylika Chauhan. StatPearls [Internet]. StatPearls Publishing, 2024. https://www.ncbi.nlm.nih.gov/sites/books/NBK563233/ 
  5. Bayram-Weston, Zubeyde, John Knight, and Maria Andrade Sienz. Nursing Times 120.3 (2024): 1-6. https://emap-moon-prod.s3.eu-west-1.amazonaws.com/wp-content/uploads/sites/3/2024/02/240226-Vitamin-B-complex-B-group-vitamins-and-their-role-in-the-body.pdf 
  6. Jayawardena, Ranil, et al. Archives of Gynecology and Obstetrics 308.4 (2023): 1075-1084. https://link.springer.com/article/10.1007/s00404-023-06925-w 
  7. Markun, Stefan, et al. Nutrients 13.3 (2021): 923. https://pmc.ncbi.nlm.nih.gov/articles/PMC8000524/#:~:text=Vitamin%20B12%20is%20often%20used,in%20such%20cases%20is%20uncertain
  8. Guéant, JL, et al. Vitamins and hormones 119 (2022): 241-274. https://www.sciencedirect.com/science/article/abs/pii/S0083672922000164 
  9. NIH Fact Sheet for Health Professionals https://ods.od.nih.gov/factsheets/VitaminB6-HealthProfessional/ 
  10. NIH Fact Sheet for Health Professionals https://ods.od.nih.gov/factsheets/VitaminB12-HealthProfessional/ 
  11. NIH Fact Sheet for Health Professionals https://ods.od.nih.gov/factsheets/Niacin-HealthProfessional/ 
  12. Hanna, Mary, et al. Perm. J 26.2 (2022): 89-97. https://pmc.ncbi.nlm.nih.gov/articles/PMC9662251/#:~:text=B%20vitamins%20act%20as%20coenzymes%20in%20several,citric%20acid%20cycle%20and%20electron%20transport%20chain
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